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KMID : 0604520060320030201
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Journal of the Society of Cosmetic Scientists of Korea
2006 Volume.32 No. 3 p.201 ~ p.208
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Antimicrobial Effect of Ethanol Extract of Dryopteris crassirhizoma Nakai on Propionibacterium acnes
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Yoon Chang-Soon
Kim Hyun-Joo
Lim Hye-Won
Choi Shin-Wook
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Abstract
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Propionibacterium acnes have been recognized as pus-forming bacteria triggering an inflammation in acne. The present study was conducted to evaluate antimicrobial activities of Dryopteris crassirhizoma Nakai against these etiologic agents of acne vulgaris and application possibility as a cosmetic resource. D. crassirhizoma crude extract and hexane fraction was prepared and its anti-acne effect against Propionibacterium acnes was investigated with minimum inhibitory concentration (MIC) and paper disk diffusion method. The MIC of D. crassirhizoma crude extract and hexane fraction was 0.008 mg/mL and 0.001 mg/mL, respectively. This implies that D. crassirhizoma extract may be an efficient anti-acne ingredient for cosmetics, as a crude extract. The paper disk diffusion assay showed that its anti-acne effect was similar to that of triclosan. The cytotoxic effect of D. crassirhizoma extract was determined by a colorimetric MTT assay using HaCaT cell line and D. crassirhizoma extract exhibited lower cytotoxic effects. Finally, we examine the stability of D. crassirhizoma extract to temperature and pH. The D. crassirhizoma extract was very stable to high temperatures (25{sim}121^{circ}C) and to wide pH range (pH; 2{sim}11), suggesting its utilization for cosmetics.
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KEYWORD
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Dryopteris crassirhizoma Nakai, antibacterial, MIC, Propionibacterium acnes, cosmetics
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